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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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p40651-EFSNS-FMR1_e1-24xms2

Plasmid

#222968

Purpose

MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence

Depositor

Xinyu Zhao

Publication

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-31CGG 5' UTR and exon1

Expression

Mammalian

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-CGG-BsmBI

Plasmid

#222966

Purpose

0CGG with BsmBI site for inserting CGG repeats

Depositor

Xinyu Zhao

Publication

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR, BsmBI site …

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-dCGG0

Plasmid

#222965

Purpose

To create 0CGG FMR1 cell line

Depositor

Xinyu Zhao

Publication

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template no CGG repeats (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-49535-sgFmr1_CGG5-2

Plasmid

#222964

Purpose

Targeting FMR1 5' UTR for CGG deletion

Depositor

Xinyu Zhao

Publication

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 sgRNA (FMR1 Human)

Use

CRISPR

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspB

Plasmid

#215400

Purpose

Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

Depositor

Gitta Coaker

Publication

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA1

Plasmid

#215398

Purpose

Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

Depositor

Gitta Coaker

Publication

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA1

Tags

6xHis Tag

Expression

Bacterial

Mutation

Codon-optimized for E. coli BL21(DE3)

Promoter

T7 promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA2

Plasmid

#215399

Purpose

Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

Depositor

Gitta Coaker

Publication

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA2

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Nterm_His

Plasmid

#220480

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1377_Nterm_His

Plasmid

#220481

Purpose

heterologous expression of PA1377 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTP489

Plasmid

#216366

Purpose

xNup155-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup155-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC263

Plasmid

#216367

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC100

Plasmid

#216369

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC99

Plasmid

#216370

Purpose

xhNup155-Nb3i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC261

Plasmid

#216371

Purpose

xhNup155-Nb3i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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