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pBLO44.5_PVYpro

Plasmid

#121634

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

chicken beta actin promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO44.3_TuMVpro

Plasmid

#121632

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

chicken beta actin promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO44.4_PPVpro

Plasmid

#121633

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

chicken beta actin promoter

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb

Plasmid

#238262

Purpose

For overexpression of ALFA-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb-KS

Plasmid

#238274

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS

Plasmid

#238270

Purpose

For overexpression of VHH05-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb-KS

Plasmid

#238263

Purpose

For overexpression of ALFA-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb

Plasmid

#238273

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGLDV_41 [11o1G]

Plasmid

#199070

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGGC_RT_Sa_sgRNA

Plasmid

#136968

Purpose

Goldengate/Greengate module for genome editing, using the Sa guideRNA.

Depositor

Klaus Theres

Insert

Sa guide RNA

Use

CRISPR

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGD253

Plasmid

#132374

Purpose

contains the sequence of mRuby2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby2

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.6_bCas9-CP1010

Plasmid

#121498

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.15_bCas9-CP1249

Plasmid

#121500

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.16_bCas9-CP1282

Plasmid

#121501

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBC2101-reci

Plasmid

#117170

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 with SV40 NLS

Use

Lentiviral

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Di-III_14_IF3_14

Plasmid

#45486

Depositor

David Baker

Article

Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:

Insert

Di-III_14_IF3_14

Tags

6xHis

Expression

Bacterial

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
Di-V_7_R3x1_7

Plasmid

#45488

Depositor

David Baker

Article

Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:

Insert

Di-V_7_R3x1_7

Tags

6xHis

Expression

Bacterial

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
Di-IV_5_PL2x2_6

Plasmid

#45487

Depositor

David Baker

Article

Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi:

Insert

Di-IV_5_PL2x2_6

Tags

6xHis

Expression

Bacterial

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
p52K-GFP-KS

Plasmid

#237690

Purpose

For overexpression of 52K-GFP-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-KS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b_msfGFP_stag_KS

Plasmid

#237630

Purpose

For expression of msfGFP_stag_KS in Ecoli

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

stag_KS

Tags

msfGFP

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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