We narrowed to 56,849 results for: plasmid
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Plasmid#135311PurposeA HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminalDepositorInsertHygR-P2A-AID
UseN terminal tagging plasmidAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
ExpressionBacterialPromotermalate dehydrogenase promoterAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
ExpressionBacterialMutationD10A & H840AAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shDyn
Plasmid#132716PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphinDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFnCpf1GG
Plasmid#131010PurposeBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of FnCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)
Plasmid#129667PurposeA repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-dCT
Plasmid#126422PurposePlasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertCalcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
TagsFLAGExpressionMammalianAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHO_pTEFmut7_CggR_R250A_ble
Plasmid#124585PurposePlasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoterDepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T4
Plasmid#125056PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertPDC1 terminator
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP4-Luc
Plasmid#124892PurposeThis plasmid contains EBNA sequence.DepositorInsertluc
Available SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga2
Plasmid#122294PurposeOverexpression for mouse Hmga2DepositorInsertHmga2 (Hmga2 Mouse)
ExpressionMammalianAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-FBFP
Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymYPET-CaURA3
Plasmid#118460PurposeYeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.DepositorInsertymYPET
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.DepositorInsertytdTomato
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)
Plasmid#114095PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (E174R/S542R/K607R)
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R, S542R and K607RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only