We narrowed to 10,136 results for: Coli

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Myc-MBP

Plasmid

#175103

Purpose

Lentiviral expression of Myc-tagged MBP

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

MBP

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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cjBlue chromoprotein

Plasmid

#117844

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, cjBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-sfGFP*- MmH-MH4R

Plasmid

#196409

Purpose

Express sfGFP with TAG and enzymes for biosynthesizing DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

sfGFP with TAG at 151 position
Tyrosine Hydroxylase (Th Mouse)
dihydropterine reductase from human (QDPR Human)
pterin-4a-carbinolamine dehydratase from human

Expression

Bacterial

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Erythropoietin

Plasmid

#182422

Purpose

Bacterial expression of mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-eythropoietin (EPO Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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meffRed chromoprotein

Plasmid

#117836

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-itis pBE-nt

Plasmid

#195343

Purpose

Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

Depositor

Alexis Komor

Article

Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

Inserts

ecTadA(8e)-SpCas9-NG
gRNA gtgcacgacgccgtatgcga

Use

CRISPR

Mutation

SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

Promoter

Lac and ProC

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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