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pT7C-DNAJB4

Plasmid

#177656

Purpose

Recombinant protein synthesis of human DNAJB4

Depositor

Jeff Kuret

Publication

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB4 (DNAJB4 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV6:T35s

Plasmid

#164741

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Publication

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr3g095180.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV2:T35s

Plasmid

#164740

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Publication

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr5g012400.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0897

Plasmid

#177090

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H

Use

Synthetic Biology

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Venus-Actb

Plasmid

#169788

Purpose

An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

C63A

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGMV-gSWEET4

Plasmid

#112796

Purpose

Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

Depositor

Humberto Prieto

Publication

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Insert

gRNA pair targeting VviSWEET4

Use

CRISPR

Expression

Plant

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-mWasabi-G

Plasmid

#164170

Purpose

Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Publication

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

tetO

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEE386

Plasmid

#149284

Purpose

T-DNA encoding TRV2 with gRNA targeting NbAG

Depositor

Daniel Voytas

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1:tNos

Expression

Plant

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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BII-C3HTK

Plasmid

#133388

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-HCA_sensor

Plasmid

#127181

Purpose

Bacterial expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Publication

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-YpODH

Plasmid

#110806

Purpose

Expresses YpODH and opine dehydrogenase from Yersinia pests

Depositor

Audrey Lamb

Publication

McFarlane et al J Biol Chem. 2018 Apr 4. pii: RA118.0020

Insert

YPO1347 (YPO1347 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0039

Plasmid

#117606

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0038

Plasmid

#117605

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCB30

Plasmid

#111910

Purpose

Expresses gRNA to target URA3 knockout locus and has KanMX marker

Depositor

Yi Tang

Insert

gRNA cassette targeting URA3 knockout locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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