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pCSII-cp PS Intein tTA Puro

Plasmid

#242027

Purpose

A plasmid to generate a stably expressing preclonal mixture using puromycin selection.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein tTA Puro

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Split PS Intein gal4-vp16

Plasmid

#242030

Purpose

Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein gal4-vp16

Expression

Mammalian

Mutation

pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBIRC5-Split PS Intein-N tTA

Plasmid

#242033

Purpose

N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein-N tTA

Tags

mTagBFP2

Expression

Mammalian

Mutation

The CMV promoter was replaced with the BIRC5 prom…

Promoter

hTERT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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phTERT-Split PS Intein-N tTA

Plasmid

#242034

Purpose

N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein-N tTA

Tags

mTagBFP2

Expression

Mammalian

Mutation

The CMV promoter was replaced with the hTERT prom…

Promoter

hTERT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Expression

Bacterial

Promoter

hns promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Expression

Bacterial

Promoter

hns promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Abr DH domain NE/AA pGEX

Plasmid

#38182

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR DH domain N282AE283A (ABR Human)

Tags

GST

Expression

Bacterial

Mutation

ABR DH domain with N282A and E283A mutations

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
Abr DH domain pGEX

Plasmid

#38181

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR DH domain (ABR Human)

Tags

GST

Expression

Bacterial

Mutation

ABR DH domain

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
human CRKL transgenic construct

Plasmid

#38186

Depositor

Nora Heisterkamp

Article

Hemmeryckx et al Cancer Res. 2001 Feb 15;61(4):1398-405.

Insert

CRKL (CRKL Human)

Use

Mouse transgenic

Mutation

Contains human CRKL introns

Promoter

human CRKL

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
myr-Abr

Plasmid

#38174

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR transcript variant 2 (ABR Human)

Expression

Mammalian

Mutation

addition of a Src myristoylation sequence (see co…

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
Abr-pLEF

Plasmid

#38157

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

ABR (ABR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
Abr GAP domain /pGEX 3X

Plasmid

#36407

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP703

Plasmid

#80000

Purpose

Mitochondria labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

cytochrome C oxidase subunit VIII (COX8A Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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phTERT-cp PS Intein tTA

Plasmid

#242032

Purpose

Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein tTA

Expression

Mammalian

Mutation

The CMV promoter was replaced with the hTERT prom…

Promoter

hTERT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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