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pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

Plasmid

#183352

Purpose

Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

TVA, EGFP and tTA

Use

AAV

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xSTOP-lox (JDW 869)

Plasmid

#224521

Purpose

A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LoxP-3xStop-LoxP

Use

Cre/Lox

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Gnaq

Plasmid

#170298

Purpose

Encoding mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A recombinant mouse Gnaq (Gnaq Mouse)

Tags

N/A

Expression

Mammalian

Mutation

Asilent mutation was introduced into the sgRNA-ta…

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagS

Plasmid

#200189

Purpose

Tom20 with reversible HaloTagS variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Tags

mEGFP and reHaloTagS

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ECD

Plasmid

#121479

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Mutation

ECD+TM

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDONOR223 R2pH-LAMP1-3xFLAG

Plasmid

#157941

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG, mCherry, and pHluorin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104095

Purpose

Expresses TREAT-siRNA reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Available Since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-jYCaMP1s

Plasmid

#135424

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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hRAD18 dC2-EGFP

Plasmid

#68826

Purpose

Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only