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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Tags

mVermilion

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro- TRF2 deltaB deltaM

Plasmid

#18013

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
PKC gamma dNPS

Plasmid

#21237

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-28

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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MLS- mir-17-19b

Plasmid

#64089

Purpose

expresses a truncated version of mir-17-92 lacking miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-17-19b (MIR17 Human)

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
MSX1-pET

Plasmid

#21024

Depositor

Corey Largman

Article

Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

Insert

MSX1 (Msx1 Mouse)

Tags

His and T7 tag

Expression

Bacterial

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only