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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-Sh-Ctrl

Plasmid

#85587

Purpose

Control hairpin sequence used in gene knockdown experiements

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

sh-Ctrl

Use

Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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DVK_AF

Plasmid

#66068

Purpose

MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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LI E-F 35S term c1

Plasmid

#54442

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Terminator of Cauliflower mosaic virus CaMV

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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Sgf73_Sgf11/PCDFDuet

Plasmid

#26964

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

sgf11 (SGF11, SGF73 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
V516 pLP-dMyc - Acceptor

Plasmid

#11712

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Available Since

July 5, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V25 pLP Flag - Acceptor

Plasmid

#11703

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

FLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V624 pDNR MCS BE - Donor

Plasmid

#11693

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pBHM2673

Plasmid

#241720

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-SpyCatcher002-MBP

Plasmid

#216295

Purpose

Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher002-MBP

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397N-UGI

Plasmid

#187173

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N-UGI

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Lifeact-mCherry

Plasmid

#185408

Purpose

Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

lifeact

Use

In vitro synthesis of mrna

Tags

mCherry

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Article

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEF:iCas9

Plasmid

#138267

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in human.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Mammalian

Mutation

Cas9-D10A, H840A

Promoter

Ef1a

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (d')

Plasmid

#99838

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (d')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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