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pET21d-colEdes3.5/Imdes3.5

Plasmid

#107111

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3.5/Imdes3.5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes4/Imdes4

Plasmid

#107112

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes4/Imdes4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes5/Imdes5

Plasmid

#107113

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes5/Imdes5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

Plasmid

#115429

Purpose

Selection for in-frame fusion expression constructs

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Type

Empty backbone

Tags

T2A-SpHIS5 and λN

Expression

Yeast

Promoter

PGK1

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP7-1/1

Plasmid

#91516

Purpose

Protein expression and purification of human SH3 domain construct MPP7-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP7-1/1 (MPP7 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO7B-1/1

Plasmid

#91405

Purpose

Protein expression and purification of human SH3 domain construct MYO7B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO7B-1/1 (MYO7B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CRKL-2/2

Plasmid

#91364

Purpose

Protein expression and purification of human SH3 domain construct CRKL-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRKL-2/2 (CRKL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF38-1/1

Plasmid

#91373

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF38-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF38-1/1 (ARHGEF38 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1F-1/1

Plasmid

#91260

Purpose

Protein expression and purification of human SH3 domain construct MYO1F-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1F-1/1 (MYO1F Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Expression

Bacterial

Promoter

T7P

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish lim1 (lhx1)

Plasmid

#74137

Purpose

Basic cDNA clone for zebrafish lim1/lhx1. Cloned in BS KS+

Depositor

Igor Dawid

Article

Toyama et al Dev Dyn. 1997 Aug;209(4):406-17.

Insert

lhx1 (lhx1b Zebrafish)

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pUG35-YDL183c-GFP

Plasmid

#70161

Purpose

yeast expression of YDL183c with GFP fusion

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

yEGFP

Expression

Yeast

Promoter

MET17

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PrbC5

Plasmid

#24787

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
Expression_vector_RO1_2

Plasmid

#158011

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_2

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_2

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_5

Plasmid

#158012

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_5

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_5

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge