164,802 results

1. pcDNA-NES-PhoCl2f-mCherry

   Plasmid

   #164048

   Purpose

   Optogenetic control of protein localization by PhoCl2f

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2f-mCherry
   

   Use

   Tags

   kozak sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Expression

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

   Plasmid

   #210857

   Purpose

   Lentiviral expression vector encoding FIP200 mutant-mCherry

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   V44D/V314D/L324D/R543D/R548D

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

   Plasmid

   #149276

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Promoter

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2CG2--3.5ubb:plin2-tdtomato

   Plasmid

   #172534

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human Parkin (1-465)

   Plasmid

   #110758

   Purpose

   Bacterial expression of human Parkin

   Depositor

   David Komander

   Article

   Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

   Insert

   PRKN (PRKN Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Use

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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