161,255 results

1. pSCW01

   Plasmid

   #72300

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CreEGFP

   Plasmid

   #165140

   Purpose

   Expression of Cre recombinase fused with EGFP in C-terminal

   Depositor

   David Threadgill, Daekee Lee

   Insert

   Cre recombinase
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-A2

   Plasmid

   #135504

   Purpose

   Mammalian expression of myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE-BI-TRIM21

   Plasmid

   #207838

   Purpose

   Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   TRIM21 (TRIM21 Human)
   mCherry
   hIgG1-FC
   VHH-GFP4
   

   Use

   Tags

   weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tTRE, hPGK

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pBabe GFP Small T antigen

   Plasmid

   #10673

   Depositor

   William Hahn

   Article

   Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

   Insert

   SV40 small T Antigen (SV40gp7 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

9. TFORF1167

   Plasmid

   #142744

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EBF3 (EBF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GALT-SEP-mRuby3

    Plasmid

    #200938

    Purpose

    trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    GalT (B4GALT1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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