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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRH3105

Plasmid

#210164

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

P194Q

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 6X PRE

Plasmid

#206168

Purpose

pMuLE L1-L4 entry plasmid containing 6 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

6X PRE

Use

Gateway cloning entry clone

Promoter

2YBTATA

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 2X PRE

Plasmid

#206166

Purpose

pMuLE L1-L4 entry plasmid containing 2 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

2X PRE

Use

Gateway cloning entry clone

Promoter

2YBTATA

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-SHmOCR

Plasmid

#206874

Purpose

Empty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusion

Depositor

Murray Junop

Article

Szabla et al Nucleic Acids Res. 2024 Jul 22:gkae635.

Type

Empty backbone

Tags

HSV + 6xHis and SuperHis (14xHis) + monomeric OCR…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Expression

Bacterial

Mutation

A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pS44i8GH-2

Plasmid

#207526

Purpose

Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP

Use

CRISPR; Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3243

Plasmid

#210187

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33P

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3217

Plasmid

#210185

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151M

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3186

Plasmid

#210181

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W353G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3178

Plasmid

#210179

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1 (ADE2 Budding Yeast)

Expression

Yeast

Mutation

G54V

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3176

Plasmid

#210178

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

G141S

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3145

Plasmid

#210169

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

D147V

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3139

Plasmid

#210167

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-HDAC5(mut-H-Y)

Plasmid

#114398

Purpose

For PYL-HDAC5 H1006Y expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006Y

Expression

Mammalian

Mutation

additional D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3135

Plasmid

#210166

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

I245N

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3057

Plasmid

#210162

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

I36D

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3046

Plasmid

#210156

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151L-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151L-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151L

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3042

Plasmid

#210152

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151E-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151E-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151E

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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