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myc-Med31 pBacPAK8

Plasmid

#15375

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med31 (Med31 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med19 pBacPAK8

Plasmid

#15376

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med19 (Med19 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED31

Plasmid

#15437

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf159

Plasmid

#12847

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf159

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf164

Plasmid

#12852

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf164

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf174

Plasmid

#12862

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf174

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf117

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf37

Plasmid

#12725

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf37

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9-mCherry-CRY2

Plasmid

#249579

Purpose

Lentiviral vector for stable expression of dCas9-mCherry-CRY2hiclu fusion protein in mammalian cells

Depositor

Tom Misteli

Article

Stortz et al J Cell Sci. 2026 Jan 19:jcs.264574. doi:

Inserts

CRY2hiclu
Sp dCas9

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Catalytically dead mutant Cas9

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pACEBac1-eIF3a-TEV-FLAG

Plasmid

#240024

Purpose

Human eIF3a for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3a (EIF3A Human)

Tags

TEV_3xFLAG

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET28_WT-LGP2 (codon optimized)

Plasmid

#248163

Purpose

Expresses full-length human LGP2 in E. coli

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis, SUMO

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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cBEST3

Plasmid

#234659

Purpose

Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

Depositor

Mingzi M. Zhang

Article

Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

Inserts

spCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3 and kasO17tss

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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K48R FLAG-HaloTag-RTEL1_DonorVector

Plasmid

#247171

Purpose

HR Donor for K48R C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.

Depositor

Thomas Cech

Article

Wu et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

K48R FLAG-HaloTag-RTEL1 (RTEL1 Human)

Promoter

Endogenous RTEL1 promoter

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO1

Plasmid

#153556

Purpose

argonaute RISC Catalytic Component 1 (AGO1 Human)

Depositor

David Bartel

Article

Briskin et al Proc Natl Acad Sci U S A. 2020 Jul 28;11

Insert

argonaute RISC Catalytic Component 1 (AGO1 Human)

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-ICP0-FxE

Plasmid

#235539

Purpose

To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0-FxE

Use

Lentiviral

Mutation

Deletion of the RING domain, ∆106-149.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIL6-Nluc-hbb

Plasmid

#239843

Purpose

Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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MKC62_HBB(HBA1reg-HBA1-2A-YFP)

Plasmid

#232408

Purpose

AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-P2A-GFP-DOX-N

Plasmid

#229407

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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