We narrowed to 17,589 results for: cript

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUN378 - pTRV2-CrGLK

Plasmid

#203888

Purpose

Viral silencing plasmid targeting the CrGLK gene from C. roseus.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK fragment

Use

RNAi

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA_F164R238

Plasmid

#216230

Purpose

S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed arginine 238 to serine, phenylalanine 168…

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_A467

Plasmid

#216234

Purpose

S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed alanine 467 to valine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB_T522

Plasmid

#216235

Purpose

S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Mutation

Changed threonine 522 to isoleucine

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NFAT5-WT

Plasmid

#215597

Purpose

For overexpression of Gal4-DBD NFAT5 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NFAT5 (NFAT5 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-TNNT2-Zeo

Plasmid

#214018

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-O-GFP

Plasmid

#205179

Purpose

Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Rat)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type rat CENP-O

Promoter

T7

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pASPIre4

Plasmid

#196656

Purpose

Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)

Expression

Bacterial

Mutation

SpeI site in Bxb1 CDS

Promoter

rhamnose-inducible promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-Halo-HSF2

Plasmid

#200198

Purpose

Expression of full length HSF2 with SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H2DBD-HR

Plasmid

#200199

Purpose

Expression of truncated HSF2 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H2DBD-HR (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-627)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H2DBD

Plasmid

#200200

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H2DBD (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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