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5HT2C-RNA2-Flag

Plasmid

#79679

Purpose

serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

serotonin receptor 2C, RNA2 (HTR2C Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-Kif2A

Plasmid

#52401

Purpose

Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

Depositor

Ryota Uehara, Gohta Goshima

Article

Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

Insert

Kif2A (KIF2A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1 D256

Plasmid

#12145

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains a deletion truncating the protein after …

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA1-FLAG

Plasmid

#79678

Purpose

serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

5HT2C-RNA1 (HTR2C Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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Cox7c-pHLuorin-sems

Plasmid

#107766

Purpose

labeling of mitochondrial cytochrome c oxidase, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit Cox7c fused to pHluorin (COX7C Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

Plasmid

#176395

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

Plasmid

#229839

Purpose

A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-MCS-c-Fos-b-globin (JDW 1237)

Plasmid

#229829

Purpose

A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

c-fos minimal promoter and b-globin intron

Use

Gateway entry clone

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CD28

Plasmid

#223596

Purpose

Lentiviral expression of human CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-beta-catenin-S33Y

Plasmid

#13371

Purpose

Retroviral expression of mouse beta-catenin S33Y mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Serine 33 to Tyrosine and Leucine 388 to Proline

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Use

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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Il12b 400bp pro-Luc

Plasmid

#20020

Depositor

Stephen Smale

Article

Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

Insert

Il12b promoter (Il12b Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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SNAP-CLC-EGFP

Plasmid

#193579

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_TRC208-SYNJ2BP-V5

Plasmid

#92149

Purpose

Expresses V5-tagged human SYNJ2BP in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP-V5 (SYNJ2BP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta Actin

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Actin-mRFP-PAGFP

Plasmid

#62382

Purpose

Expresses beta actin with both a PAGFP and mRFP tag.

Depositor

Guillaume Charras, Tim Mitchison

Article

Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

Insert

beta actin (ACTB Human)

Use

Tags

PAGFP and mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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