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TESK1 gRNA (BRDN0001145109)

Plasmid

#77892

Purpose

3rd generation lentiviral gRNA plasmid targeting human TESK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TESK1 (TESK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM6 gRNA (BRDN0001148109)

Plasmid

#77336

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM6 (TRPM6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKACB gRNA (BRDN0001147109)

Plasmid

#75961

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACB (PRKACB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKMT1A gRNA (BRDN0001149109)

Plasmid

#75880

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT1A,CKMT1B (CKMT1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p6109 pSG5-HPV31-E2(HA)

Plasmid

#24740

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 E2 (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D21A

Plasmid

#19073

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D21A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D22A

Plasmid

#19078

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 D21A/D22A

Plasmid

#19079

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

D21A/D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(130-242)

Plasmid

#19087

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(243-339)

Plasmid

#19088

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098(130-339)

Plasmid

#19090

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag-humanTRAF6 (1093)

Plasmid

#66929

Purpose

Mammalian Expression of TRAF6

Depositor

Michael Karin

Insert

TRAF6 (TRAF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Article

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pEC3109 (pSpy1C-Plac(1)_ffluc)

Plasmid

#218514

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(1)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-puro-OsAID (p5109)

Plasmid

#239359

Purpose

pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice Auxin Inducible Degradation domain

Use

Bacterial

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-humanTRAF3 (1096)

Plasmid

#66928

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Calbindin [L109/39R]

Plasmid

#177454

Purpose

Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/39.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody (CALB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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LLP109 pEF1a-gRNA-GFP

Plasmid

#100935

Purpose

GFP, puromycin and gRNA scaffold driven by EF1a promoter

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA scaffold

Use

Grna scaffold

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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