We narrowed to 9,586 results for: Coli
-
Plasmid#217821PurposeExpresses N-His-DhFld in E. coliDepositorInsertDhFld
TagsHis6xExpressionBacterialPromoterT7Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLRD20
Plasmid#208271PurposeProduction of 13R-manoyl oxide in E. coli MEV20DepositorInsertstHMGR
abCAS
cfTPS3
ExpressionBacterialMutationabCAS(D537A)Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD07
Plasmid#208260PurposeProduction of (16R)-ent-kauran-16-ol in E. coli MEV20DepositorInsertstHMGR
ptmT2
ptmT3
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTAT6
Plasmid#112594PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneTags6xHis-thioredoxinExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAT5
Plasmid#112593PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneTags6xHis-thioredoxinExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAT3
Plasmid#112584PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-fabI-gltA1-gltA2-udhA-zwf-gapAP1
Plasmid#87149PurposefabI-gltA1-gltA2-udhA-zwf-gapAP1 targeting guide RNA E. coli, Low Phosphate InductionDepositorInsertfabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB8
Plasmid#82037PurposeHybrid CitA[1-193]-Tar[204-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
Available SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB11
Plasmid#82040PurposeHybrid NarQ[1-217]-Tar[257-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
Available SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK501
Plasmid#72246PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationGln206 to Arg and changes tryptophan-151 to pheny…PromoterT7 and endogenousAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK500
Plasmid#72245PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and endogenousAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1571
Plasmid#37760DepositorInsertsptet_ompC_gfpAaV
pLac_st3 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 1569
Plasmid#37759DepositorInsertsptet_ompC_gfpAaV
pLac_micC_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD-SepRS-EFSep
Plasmid#34623DepositorExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
TagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
ExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only