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Dyn1 R271E

Plasmid

#198343

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R271E

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 R271E E720R

Plasmid

#198344

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R271E D291R

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 K44A

Plasmid

#198340

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dyn1 D406A

Plasmid

#198341

Purpose

Mammalian expression plasmid of GFP-chimera proteins.

Depositor

Justin Taraska

Article

Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pS238D1::sptse5-CT

Plasmid

#192959

Purpose

For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RG-Linker-eGFP

Plasmid

#176071

Purpose

EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

RG

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (412-618)

Plasmid

#127628

Purpose

GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

412-618 only

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11D)-mPAGFP

Plasmid

#114135

Purpose

Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cyt-AKAR5

Plasmid

#114138

Purpose

Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5 with C-terminal nuclear export signal (NES)

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11A)-mPAGFP

Plasmid

#114134

Purpose

Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B-siRNAres

Plasmid

#115333

Purpose

expresses siRNA-resistant mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

Plasmid

#65847

Purpose

Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5D-K6D

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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