We narrowed to 1,848 results for: Ipp

1. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

   Plasmid

   #83463

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4169

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

   Plasmid

   #83459

   Purpose

   Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Amino-acids 4107-4303, S4144D

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

   Plasmid

   #83457

   Purpose

   Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Amino-acids 4127-4303

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO h-PKD1 FLS L4137P-GST

   Plasmid

   #83455

   Purpose

   Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Amino-acids 4107-4303, L4137P

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO h-PKD1 FLS R4150C-GST

   Plasmid

   #83454

   Purpose

   Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Amino-acids 4107-4303, R4150C

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. tdEos-Actin-7

   Plasmid

   #57570

   Purpose

   Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. tdEos-Zyxin-6

   Plasmid

   #57696

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mKO-Vinculin-23

   Plasmid

   #57852

   Purpose

   Localization: Focal Adhesions, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pUASp-Flag-Myc-Qin-Tudor-attB

   Plasmid

   #44210

   Depositor

   Phillip Zamore

   Article

   Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

   Insert

   Qin-Tudor-domain (qin Fly)
   

   Use

   Fly transformation

   Tags

   Flag and Myc

   Expression

   Mutation

   lacks E3 domain on N-term (del aa1-274)

   Promoter

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid

    #158766

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid

    #158767

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

    Plasmid

    #179716

    Purpose

    Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV.5’VIM-eGFP-KI

    Plasmid

    #170547

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for VIM knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

    Plasmid

    #172985

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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