We narrowed to 1,203 results for: LIPI

1. pGEX-2TKN-bp1

   Plasmid

   #133428

   Purpose

   Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   SREBF1 (SREBF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SREBP1a amino acids 1-50 fused to GST

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC43-FIT2-FLL[157-159]AAA

   Plasmid

   #96994

   Purpose

   Express GFP-tagged mutated mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Amino acid residues 157-159 were mutated (FLL[157…

   Promoter

   35S

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDC32-FIT2-FLL[157-159]AAA

   Plasmid

   #96991

   Purpose

   Express mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Amino acid residues 157-159 were mutated (FLL[157…

   Promoter

   35S

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICE-EGFP-FLAG-PSMD2

   Plasmid

   #161917

   Purpose

   Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5_miniTurbo-C12orf49

   Plasmid

   #155111

   Purpose

   transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRFP-FKBP12-5ptpase domain

   Plasmid

   #67516

   Purpose

   Recruitable human type IV 5-phosphatase domain (214-644)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mutation

   INPP5E has C641A to destroy the C-terminal CAAX d…

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAD-CMV-Caveolin1-CMV-GFP

   Plasmid

   #83272

   Purpose

   Adenoviral expression of Caveolin1 with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

   Inserts

   CAV1 (CAV1 Human)
   eGFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Lyso-INPP4B-mCherry

   Plasmid

   #184055

   Purpose

   Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1). a…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA5_C12orf49-miniTurbo

    Plasmid

    #155112

    Purpose

    transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Use

    Tags

    3x Flag and miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid

    #58492

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    deleted amino acids P29 to D408 and bearing mutat…

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mRFP-FKBP12-5ptpase enzyme

    Plasmid

    #67515

    Purpose

    Recruitable human type IV 5-phosphatase enzyme

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    CMV

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pETM30-ARC105

    Plasmid

    #133426

    Purpose

    Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 5-78 fused to C-terminal of …

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICE-EGFP-FLAG-TK1

    Plasmid

    #161919

    Purpose

    Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Thymidine kinase, cytosolic (TK1 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid

    #58423

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    Deleted amino acids M1 to Y502

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LCV2_PSMB2_sgRNA_1

    Plasmid

    #155092

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMB2_sgRNA_1 (PSMB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge