We narrowed to 1,246 results for: LIPI

1. pGS_107

   Plasmid

   #160650

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_129

   Plasmid

   #160652

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

   Depositor

   Susan Lindquist, Li-Huei Tsai

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   pZ estradiol inducible promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-Rab18-sfGFP(N) HDR template

   Plasmid

   #129415

   Purpose

   HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   RAB18 HDR template (RAB18 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Peter Schultz, Jessica Seeliger

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCKO_AAVS1_sgRNA_2

   Plasmid

   #155085

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mAzami-Green-Galectin

   Plasmid

   #102419

   Purpose

   Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

   Depositor

   Geert van den Bogaart

   Article

   Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   mAzami-green

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyso-PTEN(A4)-mCherry

   Plasmid

   #184054

   Purpose

   Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-PTEN(A4)-mCherry (PTEN Human)
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1). a…

   Expression

   Mammalian

   Mutation

   Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag_HsIRE1a_deltaP29_D408_pBabePuro

   Plasmid

   #58422

   Purpose

   retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

   Depositor

   David Ron

   Article

   Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

   Insert

   IRE1a (ERN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and preprotrypsin signal sequence

   Expression

   Mammalian

   Mutation

   Deleted amino acids P29 to D408

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBIG1b_10xHisTEVATG5_ATG12

   Plasmid

   #190861

   Purpose

   Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG12 (ATG12 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-C-Flag-GK5_v2

    Plasmid

    #134282

    Purpose

    Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

    Plasmid

    #161923

    Purpose

    To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting HSD17B11 exon 1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-Blast 3XFLAG-CHP1

    Plasmid

    #173166

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304_C12orf49-V5

    Plasmid

    #155110

    Purpose

    lentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2_mClover3_AAVS1_sgRNA_2

    Plasmid

    #155099

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-parallel1-GK5_v2

    Plasmid

    #134288

    Purpose

    Expresses GK5 (isoform 2) in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagBFP2-FKBP-CYB5Atail

    Plasmid

    #108141

    Purpose

    For recruitment of ER

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mTagBFP2:FKBP1A(3-108):[GGSA]4GG:CYB5A(100-134) (CYB5A, FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CYB5A(100-134)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LCV2_AAVS1_sgRNA_3

    Plasmid

    #155089

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid

    #236225

    Purpose

    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells

    Depositor

    Chiaki Murakami

    Article

    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

    Insert

    SAMD8 (SAMD8 Human)
    

    Use

    Tags

    Hexa histidine tag (6xHis-tag) and enterokinase s…

    Expression

    Insect

    Mutation

    sterile alpha motif domain at N-terminal (1-231 b…

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

    Plasmid

    #198570

    Purpose

    SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed arginine R76 to alanine (R76A)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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