Addgene

We narrowed to 2,419 results for: gem

Showing: 1021 - 1040 of 2419 results

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pCas2B

Plasmid

#82373

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCas45

Plasmid

#82390

Purpose

sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas7

Plasmid

#82379

Purpose

dCas9 expressed from low constituve promoter, c225, in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

c225

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2E

Plasmid

#82376

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2A

Plasmid

#82372

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas39

Plasmid

#82389

Purpose

sgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas36

Plasmid

#82388

Purpose

sgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas35

Plasmid

#82387

Purpose

sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas28

Plasmid

#82385

Purpose

sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23108

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas27

Plasmid

#82384

Purpose

sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23117

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas15

Plasmid

#82383

Purpose

sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas14

Plasmid

#82382

Purpose

sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas13

Plasmid

#82381

Purpose

sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-J23108

Plasmid

#72964

Purpose

Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23108 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-J23114

Plasmid

#72965

Purpose

Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23114 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-FAM134B-LIRmut

Plasmid

#109027

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B-LIRmut (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

Promoter

EIF-1a promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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M13cp-dg3 hp

Plasmid

#218095

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

Depositor

Kevin Esvelt

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Plasmid

#129632

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1

Plasmid

#139860

Purpose

Lentiviral expression of HA-tagged human UFL1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1 (UFL1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

3XFLAG-Cas9

Expression

Mammalian

Mutation

Promoter

CBh; U6

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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