Addgene

We narrowed to 1,301 results for: pET28a

Showing: 1021 - 1040 of 1301 results

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tIVR

Plasmid

#122663

Purpose

Express tIVR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tIVR

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET28-SUMO-KaiB

Plasmid

#68050

Purpose

Strain: AM5116, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiB

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTH848-gst-disec

Plasmid

#89589

Purpose

Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

glutathione-S transferase codon-dis-optimised for bacterial expression

Use

Tags

8xhistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Dog 1-77)

Plasmid

#62060

Purpose

Expression of Dog p53 (residues 1-77) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-77

Promoter

T7

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
POC1479

Plasmid

#213805

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1480

Plasmid

#213806

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1483

Plasmid

#213809

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_canine-Caspase-1/4_enzymatic-domain

Plasmid

#183383

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head

Plasmid

#164836

Purpose

Expression of human talin2 head

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Cow 1-82)

Plasmid

#62062

Purpose

Expression of Cow p53 (residues 1-82) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-82

Promoter

T7

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (Rabbit 1-87)

Plasmid

#62058

Purpose

Expression of Rabbit p53 (residues 1-87) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-87

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET-28a-CylI

Plasmid

#48200

Purpose

Expresses CylI (type III PKS) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylI

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pA9HD

Plasmid

#8588

Depositor

Cynthia Wolberger

Article

LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

Insert

HoxA9 190-271with hexapeptide (HOXA9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pET_sfCherry(1-10)_32aalinker_sfCherry(11)

Plasmid

#83030

Purpose

Bacterial expression vector for split sfCherry screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry(1-10)_32aalinker_sfCherry(11)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBECAb-apr

Plasmid

#122001

Purpose

A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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