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Showing: 10421 - 10440 of 19455 results

  3. FUG-P2A-tC3-W

    Plasmid
    #129475
    Purpose
    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    human ubiquitin C
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129475-plasmid-map-sequence-id-384588

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  4. pSilencer2.1-U6-3JB1R+6

    Plasmid
    #190842
    Purpose
    For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190842-plasmid-map-sequence-id-379236

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  5. pSilencer2.1-U6-4JC1RR

    Plasmid
    #190847
    Purpose
    For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190847-plasmid-map-sequence-id-379268

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  6. pSilencer2.1-U6-T1-U6-AP3

    Plasmid
    #190828
    Purpose
    For mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Inserts
    T1 (an RNA aptamer of OGT)
    AP3 (an RNA aptamer of GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190828-plasmid-map-sequence-id-379201

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  7. pSilencer2.1-U6-NL1F35

    Plasmid
    #190830
    Purpose
    For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    NL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190830-plasmid-map-sequence-id-379202

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  8. pSilencer2.1-U6-NL1F50

    Plasmid
    #190831
    Purpose
    For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190831-plasmid-map-sequence-id-379228

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  9. pSilencer2.1-U6-3JB1F+2

    Plasmid
    #190833
    Purpose
    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190833-plasmid-map-sequence-id-379189

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  10. pSilencer2.1-U6-3JB1F+6

    Plasmid
    #190835
    Purpose
    For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190835-plasmid-map-sequence-id-379214

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  13. H2B-MiniR-mEm

    Plasmid
    #181990
    Purpose
    MiniR genetically encoded tag on H2B-mEmerald to be used with MiniE labeled with a reporter
    Depositor
    Kimberly Beatty
    Publication
    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-MiniR-mEm
    Tags
    mEmerald
    Expression
    Mammalian
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    181990-plasmid-map-sequence-id-357264

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  14. pMP650

    Plasmid
    #135000
    Purpose
    VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Use
    Lentiviral and Synthetic Biology
    Tags
    DpnI(aa146-254), V5 tag, and VP-64
    Expression
    Mammalian
    Mutation
    DpnI truncated to aa146-254
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135000-plasmid-map-sequence-id-267928

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  15. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173205
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173205-plasmid-map-sequence-id-339509

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  18. pLKO_S.V5.HA_NGFR

    Plasmid
    #158339
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Publication
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.HA
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158339-plasmid-map-sequence-id-330551

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  19. pLKO_S.AU1.VSVg_NGFR

    Plasmid
    #158351
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Publication
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.AU1.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158351-plasmid-map-sequence-id-330898

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  20. pLKO_Ollas.AU1.C_NGFR

    Plasmid
    #158314
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Publication
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.AU1.C
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158314-plasmid-map-sequence-id-329897

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Showing: 10421 - 10440 of 19455 results