Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 30,782 results for: PLE;

Showing: 10441 - 10460 of 30782 results

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pET23PI-Sce ESARC D218A

Plasmid

#195608

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T928C, T929C, A942C

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC

Plasmid

#195607

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

ecDHFR

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-MAFB-sg

Plasmid

#194720

Purpose

px458 with guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

Cas9 (MAFB Streptococcus pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo

Plasmid

#191398

Purpose

HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-STf

Plasmid

#182010

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Cep41-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_STf

Plasmid

#182003

Purpose

CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

[Cox8a]x2_STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_STf_KDEL

Plasmid

#182004

Purpose

CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR_STf_KDEL

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-STf

Plasmid

#182005

Purpose

CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

b4g-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmRoquin-ILLVtoEEEE_AE

Plasmid

#148636

Purpose

Mammalian Expression of DmRoquin-ILLVtoEEEE

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-ILLVtoEEEE (roq Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin1_501-1133_AE

Plasmid

#148638

Purpose

Mammalian Expression of HsRoquin1_501-1133

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_501-1133 (RC3H1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin2_AE

Plasmid

#148639

Purpose

Mammalian Expression of HsRoquin2

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin2 (RC3H2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin2_1-500_AE

Plasmid

#148640

Purpose

Mammalian Expression of HsRoquin2_1-500

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin2_1-500 (RC3H2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin2_501-1191_AE

Plasmid

#148641

Purpose

Mammalian Expression of HsRoquin2_501-1191

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin2_501-1191 (RC3H2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRoquin_1-500_AE

Plasmid

#148642

Purpose

Mammalian Expression of HsRoquin1_1-500

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_1-500 (RC3H1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmRoquin_AD

Plasmid

#148535

Purpose

Mammalian Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmRoquin_1-500_AD

Plasmid

#148536

Purpose

Mammalian Expression of DmRoquin_1-500

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_1-500 (roq Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmRoquin_501-819_AD

Plasmid

#148537

Purpose

Mammalian Expression of DmRoquin_501-819

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_501-819 (roq Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

Plasmid

#148446

Purpose

Mammalian Expression of 6xbsMS2-PolyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-PolyA-Malat

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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