We narrowed to 30,225 results for: PLE;

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPerimCh

Plasmid

#227013

Purpose

Expresses periplasmic mCherry

Depositor

Suzan Rooijakkers

Article

Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

Insert

PelB-mCherry, arabinose inducible cytosolic GFP

Expression

Bacterial

Promoter

rpsM and pBAD

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Long P2A

Plasmid

#164109

Purpose

Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM-Mt-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-pA

Plasmid

#220752

Purpose

Expression of Sun1GFP for nuclei isolation

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

Sun1-GFP-WPRE

Use

AAV

Promoter

CAG

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Short P2A

Plasmid

#164116

Purpose

Detect the short Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Short P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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crTet in pSLQ8453

Plasmid

#183965

Purpose

U6-crTet-EF1a-Puro-T2A-BFP-WPRE

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

crTet

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-iCas9-neo

Plasmid

#85400

Purpose

Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Insert

Flag-iCas9-P2A-GFP

Use

Lentiviral

Tags

Flag-iCas9-P2A-GFP

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

human PGK1

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5a

Plasmid

#221439

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5a (Fam46a Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRGEB32

Plasmid

#63142

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag7

Plasmid

#169330

Purpose

Expression of TOMM20-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag7 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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