Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,860 results for: REV

Showing: 10441 - 10460 of 18860 results

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pY71-tet.I.1

Plasmid

#133523

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_S176E

Plasmid

#131203

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 S176E (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S176E

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_Y474F

Plasmid

#131204

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 Y474F (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Y474F

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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miR-722 pCDNA

Plasmid

#97162

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-722 (mir722 Zebrafish)

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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miR-722 plix4.03

Plasmid

#97140

Purpose

Lentiviral tet-inducible expression of miR-722and Dendra 2

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-722 (mir722 Zebrafish)

Use

Lentiviral

Tags

dendra2

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317H)

Plasmid

#122269

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Histidine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325A)

Plasmid

#122274

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317Q)

Plasmid

#122271

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glutamine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D)

Plasmid

#121391

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317G)

Plasmid

#122268

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glycine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317K)

Plasmid

#122270

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to lysine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆335-417)

Plasmid

#121525

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 335-417*

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine/erg3-mCherry

Plasmid

#117993

Purpose

Co-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

erg3-mCherry

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Gal10

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(L105D)

Plasmid

#121395

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325D)

Plasmid

#121397

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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