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pHR_SFFv_4D5-WT-Highest-CAR_RHL004

Plasmid

#164826

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Plasmid

#219781

Purpose

CaST-P2A for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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LbABE8e

Plasmid

#138504

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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plenti hSyn PKM1

Plasmid

#220920

Purpose

Neuronal specific expression of PKM1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PKM1 (PKM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF2

Plasmid

#198578

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF2 (BALF2 Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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