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pSD094B

Plasmid

#218131

Purpose

Hybrid circuit displaying mmCRBN bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

Crbn (Crbn Mouse)
M13 pIII

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM166B

Plasmid

#218123

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

FRB (MTOR Human)

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-p54

Plasmid

#215844

Purpose

Expression of FLAG/HA-p54

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human p54

Expression

Mammalian

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA1.0

Plasmid

#208297

Purpose

Expresses ABE-RA1.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA1.0-SpCas9 D10A

Expression

Mammalian

Mutation

D108G

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG101

Plasmid

#189590

Purpose

To make ATG101/ATG13 HORMA complex

Depositor

James Hurley

Article

Shi et al J Cell Biol. 2020 Jul 6;219(7):e20191104

Insert

ATG101 (ATG101 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1195

Plasmid

#196027

Purpose

Complementation of MlaF-MlaE-MlaD-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Myc-mNG-3Myc

Plasmid

#190217

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Myc-mNG-3Myc

Plasmid

#190218

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-SpDcp2opt_1-242_AD

Plasmid

#148547

Purpose

Bacterial Expression of SpDcp2opt_1-242

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242 (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-CtDCP2_1-267_X

Plasmid

#148004

Purpose

Bacterial Expression of CtDCP2_1-267

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP2_1-267

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_1208-1595_M

Plasmid

#146939

Purpose

Mammalian Expression of HsTNRC6C_1208-1595

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1208-1595 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_1381-1595_M

Plasmid

#146940

Purpose

Mammalian Expression of HsTNRC6C_1381-1595

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1381-1595 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_1208-1690_L

Plasmid

#146880

Purpose

Mammalian Expression of HsTNRC6C_1208-1690

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1208-1690 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_1381-1690_L

Plasmid

#146883

Purpose

Mammalian Expression of HsTNRC6C_1381-1690

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1381-1690 (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot11_K

Plasmid

#146773

Purpose

Mammalian Expression of HsNot11

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot11 (CNOT11 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos2_X

Plasmid

#147938

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNanos2_P

Plasmid

#147202

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-AP-4 σ4

Plasmid

#198328

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-11-DmPABPC1_501-634_J

Plasmid

#146641

Purpose

Bacterial Expression of HsPABPC1_501-634

Depositor

Elisa Izaurralde

Insert

HsPABPC1_501-634

Expression

Bacterial

Mutation

2 silent mutations compared to the sequence given…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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