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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Publication

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Expression

Yeast

Promoter

GAL10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Publication

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8e-dimer

Plasmid

#138490

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGT-Ah3

Plasmid

#122570

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMAD4

Plasmid

#116791

Purpose

Lentiviral expression of SMAD4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_sfrp-1

Plasmid

#107637

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Publication

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

sfrp-1

Use

RNAi; In situ hybridization

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Cep215-FL

Plasmid

#106911

Purpose

Mammalian expression of CEP215

Depositor

Heidi Hehnly

Insert

CEP215 (Cdk5rap2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#86720

Purpose

Heat shock-induced FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phsp-16.41::FLP D5

Expression

Worm

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGCS-C6(eGFP)

Plasmid

#85729

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Publication

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

EGFP

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NTS1.0

Plasmid

#180006

Purpose

Expresses the neurotensin sensor GRAB_NTS1.0 in neurons

Depositor

Yulong Li

Insert

neurotensin sensor GRAB_NTS1.0

Use

AAV

Promoter

hSyn

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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K-SPOTIT1.0

Plasmid

#171215

Purpose

Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Publication

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

K-SPOTIT1.0

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_C

Plasmid

#168050

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLOC CMV-IRES-Dsred-blasticidin

Plasmid

#129428

Purpose

expression of Dsred-blasticidin under CMVpromoter -IRES

Depositor

Oskar Laur

Insert

Dsred-blasticidin

Use

Lentiviral

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221_SLC2A4

Plasmid

#132102

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A4 (SLC2A4 Human)

Expression

Mammalian

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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