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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

Plasmid

#127789

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

Plasmid

#127792

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg3…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

Plasmid

#119692

Purpose

For mammalian expression of LIR double mutant NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F562A/Y731A & UBD deletion (1-906 ), See depo…

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-OPTN(F178A/D474N)

Plasmid

#119694

Purpose

For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

F178A/D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJL15

Plasmid

#117430

Purpose

Expression of ARL2

Depositor

Richard Kahn

Article

Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

ARL2 (ARL2 Human)

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS2

Plasmid

#113091

Purpose

Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS1

Plasmid

#113090

Purpose

Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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NET39 Short pmRFP-N2 (451)

Plasmid

#61981

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Mutation

Short form of NET39

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only