We narrowed to 721 results for: SG

1. pAAV-PTRE-tight-hM3Dq-mCherry

   Plasmid

   #66795

   Purpose

   Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   pTIGHT promoter-hM3Dq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTIGHT TRE

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hysn-flex-dsRed-shscramble

   Plasmid

   #71383

   Purpose

   Expresses scramble shRNA Cre-dependently by flex switch

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   human synapsin promoter-flex switch-dsRed-shscramble
   

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-NHK-MYC-His6

   Plasmid

   #185350

   Purpose

   Assessing of ubiquitination in cells

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   a1-antitrypsin Hong Kong Null mutant
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   NHK mutant

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-flex-dsRed-shvgat

   Plasmid

   #67845

   Purpose

   Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   h-synapsin

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

   Plasmid

   #171150

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWPSG-Po-nCas9-BE

   Plasmid

   #195740

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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