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bPAC-mycHis_pGEM

Plasmid

#85468

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp.

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

Bacterial Photoactivated Adenylyl Cyclase

Use

Tags

His and Myc

Expression

Mutation

Promoter

T7 promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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B0015_DE

Plasmid

#66035

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0015_DF

Plasmid

#66036

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0015_DG

Plasmid

#66037

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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B0015_DH

Plasmid

#66038

Purpose

MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Double Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-MYB

Plasmid

#51305

Purpose

Expression of MYB after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-myb avian myeloblastosis viral oncogene homolog (MYB Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET-20b-M-Ntx-S1

Plasmid

#58716

Purpose

Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

Methionine

Expression

Bacterial

Mutation

Changed Asparagine 1 to Serine

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

n/a

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCK354

Plasmid

#129698

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120010799

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

Plasmid

#114378

Purpose

expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-KA2N-EGFP (NEWENTRY )

Use

AAV

Tags

EGFP and KA2N

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-Nvec-codonopti-Cas9-2xNLS

Plasmid

#141108

Purpose

Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

Depositor

Matthew Gibson

Article

Hill et al Methods Mol Biol. 2022;2450:437-465. doi

Insert

Nematostella codon optimized Cas9

Use

Unspecified

Tags

Expression

Mutation

All amino acids coded for by codons optimized for…

Promoter

T7

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Tags

Expression

Mutation

K271Q

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCK351

Plasmid

#129696

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120034435

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

Plasmid

#114371

Purpose

expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-MBD (NEWENTRY )

Use

AAV

Tags

EYFP and MBD

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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