We narrowed to 24,459 results for: Spr

1. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

   Plasmid

   #240799

   Purpose

   CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

   Depositor

   Hans Clevers

   Article

   Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

   Insert

   mNeonGreen-mCherry-CAAX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

   Plasmid

   #240800

   Purpose

   CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

   Depositor

   Hans Clevers

   Article

   Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

   Insert

   mNeonGreen mCherry-CAAX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   PGK

   Available Since

   Aug. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

   Plasmid

   #197507

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

   Plasmid

   #197508

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

   Promoter

   CMV and T7

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

   Plasmid

   #164041

   Purpose

   CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   mCherry-CRISPR-TagV2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

   Plasmid

   #133560

   Purpose

   Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

   Plasmid

   #170808

   Purpose

   PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Inserts

   GID1-VPR-IRES-HygR
   GAI-tagBFP-Sa_dCas9 (GAI S. aureus, Synthetic, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   Synonymous mutation in PYL1 to remove XhoI site (…

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164042

    Purpose

    CRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

    Plasmid

    #159581

    Purpose

    Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75234

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75235

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75238

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75239

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

    Plasmid

    #221819

    Purpose

    Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    GFP11-HA donor with homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

    Plasmid

    #209923

    Purpose

    A knock-out vector for the dog CTNND1

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    A gRNA targeting the dog CTNND1 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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