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pGGC-PEA3AT

Plasmid

#210536

Purpose

Cloning P. sativum PEA3A terminator as GreenGate module C

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

PEA3At

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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INT_GFPsp

Plasmid

#212144

Purpose

Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23119

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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INT_GFPsp_CamRcargo

Plasmid

#212143

Purpose

Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23119

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-EE

Plasmid

#198298

Purpose

Produces PVCs retargeted with EE tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-EE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0013

Plasmid

#205582

Purpose

NpuDnaE(C)_HYG(53-341)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0011

Plasmid

#205580

Purpose

NpuDnaE(C)_KAN(193-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0010

Plasmid

#205579

Purpose

KAN(1-192)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0009

Plasmid

#205578

Purpose

NpuDnaE(C)_KAN(132-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0008

Plasmid

#205577

Purpose

KAN(1-131)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0019

Plasmid

#205992

Purpose

pNpuDnaE(C)_HygR(53-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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nNc-ABE

Plasmid

#194095

Purpose

CMV-TadA 8e-nNcCas9. A-to-G base editing in human cells.

Depositor

Zhanjun Li

Article

Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

Insert

TadA 8e-nNcCas9

Use

Tags

Expression

Mammalian

Mutation

NcCas9 D16A

Promoter

CMV

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pIN1_GGGSx3_mScarlet

Plasmid

#182936

Purpose

C-terminal mScarlet with flexible linker (GGGGS)x3

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1108_Tier3(Lenti_inverse)

Plasmid

#169663

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge