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mCh-2xFKBP (no insert) (pBS1139)

Plasmid

#185322

Purpose

The empty vector used for the FKBP experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only
empty-mCh-SspB (pBS1140)

Plasmid

#185323

Purpose

The empty vector used for the iLID-SspB experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AfrLEA2 (pBS0910)

Plasmid

#185284

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA2

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_CsLEA11 (pBS0786)

Plasmid

#185255

Purpose

For the mammalian expression of the Cucumber protein DHN_CsLEA11. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_CsLEA11

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_AmDHN132 (pBS0781)

Plasmid

#185253

Purpose

For the mammalian expression of the Ammopiptanthus protein DHN_AmDHN132. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_AmDHN132

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_TJ190_negative (pBS0773)

Plasmid

#185250

Purpose

For the mammalian expression of the synthetic protein TJ190_negative. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

TJ190_negative

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_SAHS4_k1_0 (pBS0769)

Plasmid

#185249

Purpose

For the mammalian expression of the tardigrade protein SAHS4_k1_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SAHS4_k1_0

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1386-1535_S

Plasmid

#147540

Purpose

Mammalian Expression of HsNot1iso1_1386-1535

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1386-1535 (CNOT1 Human)

Expression

Mammalian

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMtb MCM

Plasmid

#138296

Purpose

Expression of Mtb MCM small and large subunit

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Inserts

mutA
mutB

Tags

His tag and TEV cleavage site

Expression

Bacterial

Mutation

inserted TEV cleavage site between His-tag and m…

Promoter

T7

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/ TO myc-cCE-bio (cCE-bio)

Plasmid

#82473

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)

Tags

TEV-Biotin and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F07

Plasmid

#71056

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mi-2 (Mi-2 Fly)

Use

Gateway entry vector

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase delta 1278-1291

Plasmid

#59830

Purpose

Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 1278-1291

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PT7-alphaHL

Plasmid

#227650

Purpose

pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.

Depositor

Richard Murray

Insert

alpha-hemolysin

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLgw V5-EcoDam

Plasmid

#59210

Purpose

Mammalian DamID control lentiviral vector for expression of V5-Dam only

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

EcoDam

Use

Lentiviral; Damid

Tags

V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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