178,384 results
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Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pTarget_CAST
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-V5-miniTurboID-NES
Plasmid#116905PurposeExpresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiTurboID
UseDrosophila expressionAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralMutationWTPromoterU6Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
ExpressionBacterialPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
TagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only