We narrowed to 28,953 results for: Tat

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NMRK1 gRNA (BRDN0001144748)

Plasmid

#75829

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK1 (NMRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STYK1 gRNA (BRDN0001146987)

Plasmid

#75697

Purpose

3rd generation lentiviral gRNA plasmid targeting human STYK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STYK1 (STYK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5-WT-FRAG

Plasmid

#68873

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa

Promoter

tac

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pZS1[GalS-L,RbsR-L]

Plasmid

#60763

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-L,RbsR-L]

Plasmid

#60761

Purpose

Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,TreR-L]

Plasmid

#60766

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIB-FLAG-hDIXDC1-L-S592A

Plasmid

#61225

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-Hygro-FLAG-hDIXDC1 S592A

Plasmid

#61214

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-Blast-FLAG-hDIXDC1 S592A

Plasmid

#61221

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Promoter

PGK

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A

Plasmid

#61219

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Promoter

CMV/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCR2.1-Ins2-842

Plasmid

#53969

Purpose

842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

mouse Ins2 gene partial (Ins2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 shMut m7

Plasmid

#52037

Purpose

encodes shMut HMGA2 m7

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, all seven let-7 sites…

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut ATG wt

Plasmid

#52038

Purpose

encodes shMut HMGA2 ATG wt

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

shRNA binding site mutated, first ATG mutated

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L57E/L113E/W284A

Plasmid

#47360

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L57E/L113E/W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 4

Plasmid

#45387

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated from agacaaggttgt to acactaggatga

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only