We narrowed to 12,202 results for: 112

1. tet-pLKO.1_puro_shJUND #1

   Plasmid

   #136581

   Purpose

   Expresses an inducible short hairpin targeting human JUND sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shJUNDv1 (JUND Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-B2UP49_GFP

   Plasmid

   #103071

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pFUW-TetO-LITAF

   Plasmid

   #178444

   Purpose

   doxycycline-inducible expression of Human LITAF in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   LITAF (LITAF Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti-UBC- IKZF5-3xHA-pGK-PUR

   Plasmid

   #107385

   Purpose

   Lentiviral expression of IKZF5-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF5 (IKZF5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

   Plasmid

   #184406

   Purpose

   yeast surface display of the SARS-CoV-2 Beta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   K417N+E484K+N501Y

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

   Plasmid

   #62577

   Purpose

   Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCHKU38.2-2.2

   Plasmid

   #110670

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Expression

   Yeast

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MO70-N-Flag-hCRACR2A-isoform-a-Q604L

   Plasmid

   #79595

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

   Insert

   CRACR2A-isoform-a (CRACR2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q604L

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. MO70-N-Flag-hCRACR2A-isoform-a-T559N

    Plasmid

    #79594

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

    Insert

    CRACR2A-isoform-a (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T559N

    Promoter

    CMV

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMXs_FLAG-MCART1W283A

    Plasmid

    #138409

    Purpose

    Express FLAG-tagged MCART1W283A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, W2…

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO/SH/GW-Mst2

    Plasmid

    #84300

    Purpose

    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

    Depositor

    Jonathan Chernoff

    Article

    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

    Insert

    Mst2 (STK3 Human)
    

    Tags

    HA, SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN_TTmiRc2_BirA_CDKN1A

    Plasmid

    #136523

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Entry vector for gateway cloning

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSR:MSMEG_0709:Dendra

    Plasmid

    #159077

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0709
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag MFF iso1 S172A

    Plasmid

    #74391

    Purpose

    Flag tagged human S172A MFF isoform 1 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26) and Ser 172 Ala.

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

    Plasmid

    #69050

    Purpose

    lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    Q147H

    Promoter

    CAG

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. RalA (G23V)( full )-pcw107-V5

    Plasmid

    #64645

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    G23V

    Promoter

    PGK

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

20. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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