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pNC-Amp-GFP-N

Plasmid

#193416

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-LaminB1

Plasmid

#184448

Purpose

Nuclear memebrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-CAAX

Plasmid

#184447

Purpose

Plasma membrane-targeted TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-NLS

Plasmid

#184450

Purpose

Nucleus-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-LaminB1

Plasmid

#184452

Purpose

Nuclear memebrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-NES

Plasmid

#184461

Purpose

Cytosolic EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mEGFP]mp-mCherry2

Plasmid

#182870

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mEGFP, myr-palm-mCherry2

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-AUR1-msGFP2

Plasmid

#177101

Purpose

Expresses Aur1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

AUR1 (AUR1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-RBD2-6xmGFP

Plasmid

#177109

Purpose

Expresses Rbd2-6xmEGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

RBD2 (RBD2 Budding Yeast)

Tags

6xmEGFP

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC2-msGFPx3

Plasmid

#177110

Purpose

Expresses Sec2-msGFPx3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SEC2 (SEC2 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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BB1_23_MS2bind_VP64_SV40NLS

Plasmid

#161479

Purpose

BB1 encoding a MS2 binding VP64 fusion protein with an additional SV40 NLS

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Insert

MS2 binding protein + VP64 activation domain

Use

Synthetic Biology

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKM634

Plasmid

#134219

Purpose

Baculovirus expression of CENP-HIKM

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP HIKM

Expression

Insect

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pKM672

Plasmid

#134221

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 1-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-419

Plasmid

#104720

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MPK3 (MPK3 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-22

Plasmid

#104708

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 W40A (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

W40A, BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-131

Plasmid

#104713

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MIEL1 (AT5G18650 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-489

Plasmid

#104715

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

NEDD8 (RUB1 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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