Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 62,935 results for: cloning

Showing: 10661 - 10680 of 62935 results

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pUC19_CoV-2_frD_ORF7b-3xFLAG

Plasmid

#253137

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), 3xFLAG C-terminal of orf7b

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF7b-3xFLAG (ORF7b SARS-CoV-2)

Tags

3X FLAG

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_3xFLAG-ORF7a

Plasmid

#253136

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), 3xFLAG N-terminal of orf7a

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_3xFLAG-ORF7a (ORF7a SARS-CoV-2)

Tags

3X FLAG

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ΔORF3a67a8

Plasmid

#253131

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, orf6, orf7a and orf8

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ΔORF3a67a8

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF6-BirA

Plasmid

#253132

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), BirA fused to orf6, C-terminal

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF6-BirA (ORF6 SARS-CoV-2)

Tags

BirA

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF67a8

Plasmid

#253130

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf6, orf7a and orf8

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF67a8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF8

Plasmid

#253128

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf8

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF7b

Plasmid

#253127

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf7b

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF7b

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF7a

Plasmid

#253126

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf7a

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF7a

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF6

Plasmid

#253125

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf6

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF6

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ΔORF3a

Plasmid

#253124

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D SARS-CoV-2 Δorf3a

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔORF68

Plasmid

#253129

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), deletion of orf6 and orf8

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔORF68

Expression

Bacterial

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD1_XBB.1.5 S

Plasmid

#253119

Purpose

For recombinant virus rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Omicron isolate XBB.1.5 Spike

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron XBB.1.5 spike

Expression

Bacterial

Mutation

S: F1089F

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCM0-109

Plasmid

#218200

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCM0-101

Plasmid

#218199

Purpose

Multi-Stop for adding 3 stops for each frame

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Multi-Stop

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCM0-099

Plasmid

#218201

Purpose

Strep-8xHis-tag for protein detection and purification

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-8xHis

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCM0-100

Plasmid

#218202

Purpose

3xHA epitope tag for protein detection

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xHA

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-723

Plasmid

#218203

Purpose

1xHA-8xHis-tag for protein detection and purification

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

1xHA-8xHis

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM0-054

Plasmid

#218204

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-871

Plasmid

#218019

Purpose

8xHis-1xHA-tag for protein detection and purification

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-1xHA-tag

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-1022

Plasmid

#218020

Purpose

8xHis-SP20-3xHA-tag for protein detection and purification

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-SP20-1xHA-tag

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10661 - 10680 of 62935 results

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