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pSG5HA-MAGE-KA240,245

Plasmid

#89140

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

K240A, K245A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom27bp-pGL4.10Luc

Plasmid

#60876

Purpose

Luc reporter driven by 27 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pWPXL-resNP1

Plasmid

#65061

Purpose

Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

G447A, C450T, A451T, G452C, C454A, C456A, C459G

Promoter

EF1alpha

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Luc-Hdm4-3'UTR-F2d5

Plasmid

#64018

Purpose

Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

Depositor

Lin He

Article

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

HDMX (MDM4 Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL C214S

Plasmid

#47086

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234A_S235A

Plasmid

#40634

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Mammalian

Mutation

T234A_S235A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234E_S235E

Plasmid

#40635

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E_S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234E

Plasmid

#40636

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234A

Plasmid

#40637

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

T234A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_S235E

Plasmid

#40638

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
vipr1_L (OZ559)

Plasmid

#28084

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting vipr1 (LOC100334247 Zebrafish)

Use

Zebrafish targeting

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only
vipr1_R (OZ560)

Plasmid

#28085

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting vipr1 (LOC100334247 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pGEX4T-1-mMcl-1(T144A)

Plasmid

#25382

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(T144A) (Mcl1 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

changed threonine 144 to alanine

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
p6111 pSG5-HPV31-KWK-E8^E2C(HA)

Plasmid

#24742

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 KWK E8^E2C (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Mutation

residues 5 to 8 from KWK to AEA

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A

Plasmid

#20233

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only