19,834 results

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pTK305

Plasmid

#66812

Purpose

IVT vector for Fluc mRNA

Depositor

Ron Weiss

Article

Andries et al J Control Release. 2015 Sep 2. pii: S016

Insert

Firefly luciferase

Use

Luciferase; Mrna ivt

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAmCyan1-C1-ADCY6

Plasmid

#113905

Purpose

N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 (ADCY6 Human)

Tags

Cyan

Expression

Bacterial and Mammalian

Promoter

pCMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Tubp-Gal4 in pCaSpeR4

Plasmid

#17747

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL4 (GAL4 Budding Yeast)

Tags

Tubulin 1alpha promoter and hsp70 PolyA

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pC4-RhE-FRB-Fis1

Plasmid

#68056

Purpose

express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

Fis1 (FIS1 Human)

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET28-SpRY-NLS-6xHis (gbr2101)

Plasmid

#181743

Purpose

bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

bacterial codon optimized SpRY

Use

In vitro transcription; t7 promoter

Tags

BPNLS(SV40)-6xHis and MKIEE MBP tag

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

T7

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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VCP(DK0)-EGFP

Plasmid

#23974

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

E305QE578Q

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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p4947 pcDNA4c hBrd4 full-length

Plasmid

#14441

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-GRASP55

Plasmid

#137708

Purpose

Eukaryotic expression of rat GRASP55 full-length

Depositor

Yanzhuang Wang

Insert

GRASP55

Tags

EGFP

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_WT

Plasmid

#81856

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

Plasmid

#89060

Purpose

AAV-gRNA cloning vector with GFP reporter

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_M18

Plasmid

#82356

Purpose

Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

hEF1-HTLV prom

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Tags

His8 + SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pMXsIP GFP-VPS33A

Plasmid

#67022

Purpose

human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
YFP-Parkin-IRES-zeo

Plasmid

#61728

Purpose

Expression of human Parkin fused to eYFP

Depositor

Douglas Green, Stephen Tait

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

Plasmid

#102886

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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