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pBABE-EGFP-THAP11-rescue-3xFLAG

Plasmid

#37000

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pTFG011_Spike_Parental_D614G_3xFlag

Plasmid

#191571

Purpose

SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G mutation. Last 19 amino acids truncated.

Promoter

CMV

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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PTRF/Cavin1_R (OZ514)

Plasmid

#27189

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-Blast ACSL4

Plasmid

#173164

Purpose

Retroviral vector to express sgRNA resistant human ACSL4

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin XLone-eGFP

Plasmid

#159754

Purpose

AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

all-in-one tet-on system

Use

CRISPR and TALEN; Donor plasmid for targeted knoc…

Expression

Mammalian

Promoter

TRE3GS inducible promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG2IG-Hmga2

Plasmid

#122294

Purpose

Overexpression for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-3z/601

Plasmid

#26656

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Insert

Expression

Bacterial

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-human TMEM217-PA

Plasmid

#249410

Purpose

Expression vector of human TMEM217 isoform 3 tagged with PA at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 isoform 3 (TMEM217 Human)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry-NLS

Plasmid

#251133

Purpose

Gachapin-A with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4B-P2A-EBFP2-NLS

Plasmid

#251134

Purpose

Gachapin-B with a transfection marker EBFP2-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4B

Tags

P2A-EBFP2-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast POLE

Plasmid

#241437

Purpose

Expresses DOX-suppressible POLE

Depositor

Richard Possemato

Insert

POLE (POLE Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Silent Mutations disrupt shRNA targeting sites

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_BSD_donor

Plasmid

#246394

Purpose

PHF1 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-p53RE(CDKN1A)-CMVmin-mScarlet-I-NLSx3-AU1-PGKpuro

Plasmid

#241845

Purpose

Expresses p53 transcription fluorescence reporter in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

CDKN1A promoter (CDKN1A Human)

Tags

mScarlet-I-3xNLS

Expression

Mammalian

Mutation

CDKN1A promoter (1015 bp) + CMV minimal promoter …

Promoter

n/a

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet_6xHis-TEV-mCherry

Plasmid

#227710

Purpose

Expression of recombinant protein for purification (used as control)

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SARS-CoV-2-NSP3-V5

Plasmid

#214429

Purpose

Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SARS-CoV-2 NSP3 (part of SARS-CoV-2 ORF1ab) (ORF1ab )

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP3-VA

Plasmid

#191214

Purpose

Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 3 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227896

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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