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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Plasmid

#238282

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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SNX29-PX (269-391)

Plasmid

#119108

Purpose

Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX29-PX (269-391)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.L276V.K312A

Plasmid

#238945

Purpose

Green glucose sensor, moderate sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-TCOF1-gRNA

Plasmid

#237633

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 gRNA (TCOF1 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX Myc-HA

Plasmid

#229499

Purpose

For lentiviral expression of Myc coding sequence with an HA tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

MYC

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.USH2A

Plasmid

#226555

Purpose

Minigene encoding USH2A exon 13 and surrounding introns/exons

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

USH2A (USH2A Human)

Expression

Mammalian

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW100003

Plasmid

#222442

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

mCherry2-HyP5SM

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111114

Plasmid

#222459

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111118

Plasmid

#222461

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111113

Plasmid

#222458

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111101

Plasmid

#222452

Purpose

Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR::Cre::NOS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_703128

Plasmid

#224394

Purpose

For expression of the type I CBASS system encoded by Escherichia coli 703128

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_BWH59

Plasmid

#224393

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BWH59

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

2TM_EcCdnD

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-OST4-mCherry (EcoRV) (XH26)

Plasmid

#206475

Purpose

Expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-X-hPGK-PuroR-Z

Plasmid

#218904

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

hPGK-PuroR

Expression

Mammalian

Mutation

246T>A and 398T>C

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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actb2-SecAnxaV-mKate2-acry-EGFP

Plasmid

#123628

Purpose

Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

AnnexinA5

Tags

mKate2

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_GpA_wt

Plasmid

#207842

Purpose

BLaTM-System GpA wt positive control in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pnCas9-AID

Plasmid

#196242

Purpose

Plasmid expressing the nCas9-AID fusion

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

nickase Cas9 fused to AID

Use

CRISPR

Expression

Bacterial

Promoter

tac promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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