We narrowed to 15,679 results for: Nes

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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D10A-Cas9

Plasmid

#239383

Purpose

For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A

Use

CRISPR

Tags

BPNLS and SV40 NLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 S. pyogenes, Synthetic)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A-AtUBI10-dCas9-24XGP41

Plasmid

#202011

Purpose

Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Promoter

AtUBI10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYihI-N-term K-R

Plasmid

#233091

Purpose

Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI with N-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

N-terminal lysine residues mutated to arginine

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmcb306-GFP-Rab12-mito

Plasmid

#207349

Purpose

Mammalian expression of Rab12-mito

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab12-mito (RAB12 Human)

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ20 PPM1H

Plasmid

#207336

Purpose

Mammalian expression of HA-Δ20 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ20

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ34 PPM1H

Plasmid

#207337

Purpose

Mammalian expression of HA-Δ34 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ34

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37 PPM1H

Plasmid

#207338

Purpose

Mammalian expression of HA-Δ37 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ44 PPM1H

Plasmid

#207339

Purpose

Mammalian expression of HA-Δ44 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ44

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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