We narrowed to 15,772 results for: LEA

1. ANKRA

   Plasmid

   #32877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA (ANKRA2 Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

2. SPL11p::pCGTAG (SPL11p::NLS-GFP)

   Plasmid

   #27411

   Depositor

   David Bartel

   Article

   Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

   Insert

   SPL11 promoter (AT1G27360 Mustard Weed)
   

   Mutation

   SPL11 promoter transcriptionally fused to a nucle…

   Available Since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIC.B4.BSU37660

   Plasmid

   #11535

   Depositor

   Sung-Hou Kim

   Article

   Xu et al J Struct Funct Genomics. 2005 Dec;6(4):2

   Insert

   phosphotransacetylase
   

   Tags

   HisTag-MBP, TEV cleavable

   Expression

   Bacterial

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CalfluxCTN

   Plasmid

   #190068

   Purpose

   Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

   Depositor

   Carl Johnson

   Article

   Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

   Insert

   CalfluxCTN
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP1112

   Plasmid

   #104158

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTP396

   Plasmid

   #149336

   Purpose

   Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

   Depositor

   Rebecca Voorhees

   Article

   Pleiner et al Science. 2020 May 21. pii: science.abb50

   Insert

   anti-GFP nanobody
   

   Tags

   Biotin acceptor peptide (Avi-tag), His14, and SUM…

   Expression

   Bacterial

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. bZiPro

    Plasmid

    #86846

    Purpose

    express NS2B-NS3 protease in E.coli

    Depositor

    Dahai Luo

    Article

    Zhang et al Science. 2016 Dec 8. pii: aai9309.

    Inserts

    NS3 1-177
    NS2B45-96
    

    Tags

    a hexahistidine tag and a thrombin cleavage site

    Expression

    Bacterial

    Mutation

    NS2B cofactor region (residues 45–96) and NS3 pro…

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-3C-sGFP11_pcDNA3.1(-)

    Plasmid

    #182932

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 11, FLAG tag, and HA signa…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET16better_H10_HRV3C_chitinase

    Plasmid

    #175091

    Purpose

    Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

    Depositor

    Jay C Dunlap

    Article

    Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

    Insert

    chitinase
    

    Tags

    10X His, HRV-3C

    Expression

    Bacterial

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyCatcher003

    Plasmid

    #133447

    Purpose

    Expresses SpyCatcher003 in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Lyn-AKAR4

    Plasmid

    #61620

    Purpose

    Lipid raft-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    Lyn-AKAR4
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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