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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Pigb-V5

Plasmid

#175160

Purpose

Lentiviral expression of mouse Pigb-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Pigb (Pigb Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem209

Plasmid

#175154

Purpose

Lentiviral expression of V5-tagged mouse Tmem209

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem209 (Tmem209 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33-PA

Plasmid

#179129

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hSPATA33-8xHIS-1D4

Plasmid

#179133

Purpose

Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (SPATA33 Human)

Tags

1D4 and 8XHIS

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Zdhhc6-V5

Plasmid

#175146

Purpose

Lentiviral expression of mouse Zdhhc6-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Zdhhc6 (Zdhhc6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-hPMEL

Plasmid

#174607

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#2

Plasmid

#163389

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#1

Plasmid

#163388

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#3

Plasmid

#163390

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSunS 1-335

Plasmid

#171673

Purpose

SunS 1-335 expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_stgRNA-Puro

Plasmid

#164124

Purpose

Backbone for lentiviral stgRNA library construction (library 1 and 2)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_S

Plasmid

#163086

Purpose

Swingshift mutant FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

Fah_S
Fah_S

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAshort

Plasmid

#153547

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 603-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-5U6-sgRNAs-hsyn-EGFP

Plasmid

#112213

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

sgRNAs

Use

AAV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-1.8k-Venus-WPRE

Plasmid

#156398

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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