We narrowed to 1,254 results for: sumo

1. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208041

   Purpose

   Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   TurboID
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BLM DM

   Plasmid

   #80071

   Purpose

   Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

   Depositor

   Nathan Ellis

   Article

   Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   GFP-BLM

   Expression

   Mammalian

   Mutation

   K317R (AAA>AGA) and K331R (AAA>AGA)

   Promoter

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-PIAL2M-wt

   Plasmid

   #67909

   Purpose

   Inducible expression of PIAL2 in E.coli

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496

   Promoter

   P-lac

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MZB186

   Plasmid

   #228489

   Purpose

   Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Type

   Empty backbone

   Use

   Tags

   10xHis-SUMO(Smt3)-Avitag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTNS2

   Plasmid

   #87802

   Purpose

   For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS1

   Plasmid

   #87797

   Purpose

   For constructing SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTMS1

   Plasmid

   #87799

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-hTDG-PreE2

    Plasmid

    #52272

    Purpose

    Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Use

    Tags

    Ubc9-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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