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Showing: 1061 - 1080 of 2723 results

  5. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  10. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  13. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

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  16. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Wensheng Wei
    Article
    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213965-plasmid-map-sequence-id-422887

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  17. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid
    #198240
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198240-plasmid-map-sequence-id-393627

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Showing: 1061 - 1080 of 2723 results