We narrowed to 12,362 results for: NUC

1. pT7-7 asyn S87A/S129E

   Plasmid

   #36065

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein S87A/S129E (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S87A/S129E

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK610

   Plasmid

   #72531

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK540

   Plasmid

   #72446

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK543

   Plasmid

   #72516

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains additional Ala2

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOpen-RNAse A

   Plasmid

   #165565

   Purpose

   Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   RNAse A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pAAV asyn S129A

   Plasmid

   #36066

   Depositor

   Patrick Aebischer, Hilal Lashuel

   Insert

   alpha-synuclein S129A (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S129A

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. GST-MNase

   Plasmid

   #136291

   Purpose

   Expresses Micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV hSyn GFP-Cre

   Plasmid

   #175381

   Purpose

   expresses GFP-Cre under the control of a human synapsin promoter

   Depositor

   Jason Aoto

   Insert

   GFP-Cre recombinase
   

   Use

   AAV

   Tags

   Cre is fused to GFP. GFP is fused to additional n…

   Expression

   Mutation

   Promoter

   human synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHMGWA-Pa14Csy4H29A

   Plasmid

   #41092

   Depositor

   Jennifer Doudna

   Article

   Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

   Insert

   Csy4
   

   Use

   Tags

   His6-MBP

   Expression

   Mutation

   His29Ala mmutation to abolish ribonuclease activi…

   Promoter

   T7

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pEQ111m

    Plasmid

    #40190

    Depositor

    Paul Modrich

    Article

    Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Glutamic Acid 111 to Glutamine

    Promoter

    Lambda PL

    Available since

    Nov. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAAV asyn S129D

    Plasmid

    #36069

    Depositor

    Patrick Aebischer, Hilal Lashuel

    Insert

    alpha-synuclein S129D (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129D

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pAAV asyn S129E

    Plasmid

    #36068

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129E (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129E

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

13. Sticky-355-3X

    Plasmid

    #159644

    Purpose

    Non-coding DNA sequence for dinucleosome preparation

    Depositor

    Curt Davey

    Article

    Adhireksan et al Nat Commun. 2020 Sep 21;11(1):4747. doi:

    Type

    Empty backbone

    Use

    Bacterial vector for dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sticky-349-3X

    Plasmid

    #159643

    Purpose

    Non-coding DNA sequence for dinucleosome preparation

    Depositor

    Curt Davey

    Article

    Adhireksan et al Nat Commun. 2020 Sep 21;11(1):4747. doi:

    Type

    Empty backbone

    Use

    Bacterial vector for dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV asyn S129G

    Plasmid

    #36067

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129G (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129G

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAdTrack-PPARdelta

    Plasmid

    #16529

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Insert

    PPAR delta (PPARD Human)
    

    Use

    Adenoviral

    Tags

    2x HA (YDVPDYAS)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEcoFus

    Plasmid

    #187833

    Purpose

    1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

    Depositor

    Jörg Vogel

    Article

    Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4134336

    Plasmid

    #152014

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4134905

    Plasmid

    #151966

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (Nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (Nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. p3-K230-FokI_L (wt)

    Plasmid

    #32544

    Depositor

    Jin-Soo Kim

    Article

    Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

    Insert

    ZFN
    

    Use

    Tags

    HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2011

    Availability

    Academic Institutions and Nonprofits only