Addgene

We narrowed to 21,630 results for: Sis

Showing: 1061 - 1080 of 21630 results

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pICSL90016

Plasmid

#117514

Purpose

Cas9_2 Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_2:GCTT:BsaI

Use

CRISPR

Tags

NLS_SV40

Expression

Plant

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ358

Plasmid

#117505

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtRPS5a

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CsCYP722C(pYL877)

Plasmid

#178296

Purpose

Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CsCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 mu

Plasmid

#44938

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 mu (GRF9 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
SPL10p::pCGTAG (SPL10p::NLS-GFP)

Plasmid

#27410

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 promoter (AT1G27370 Mustard Weed)

Use

Tags

Expression

Mutation

SPL10 promoter transcriptionally fused to a nucle…

Promoter

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pZA70

Plasmid

#174784

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbJIM2

Use

RNAi

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Use

Tags

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD111

Plasmid

#172957

Purpose

pRMCD77 encoding Wzz mutant G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G311A mutant gene

Use

Tags

Expression

Bacterial

Mutation

D168G and N33D- please see depositor comment

Promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Use

Tags

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_STS:P4S

Plasmid

#139797

Purpose

expression of biosynthetic enzyme STS in fusion with CC segment P4S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS:P4S

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pJK349

Plasmid

#71566

Purpose

Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK272

Plasmid

#71562

Purpose

Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid acetylenase

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP-S95A

Plasmid

#48245

Purpose

Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

Expression

Bacterial

Mutation

Changed Serine 95 to Alanine

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA003

Plasmid

#48814

Purpose

Provides the WUS promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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zw4 (egghead) cDNA#7

Plasmid

#37398

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
zw4 (egghead) cDNA#4e

Plasmid

#37399

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

miR156 target sites of SPL11 mutated

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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6mpSPL10F13R13::pBIB-BAS (6mSPL10)

Plasmid

#27408

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 mutant (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Tags

Expression

Mutation

miR156 target sites of SPL10 mutated

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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